The carboxylic acid needs to be activated by certain carbodiimide such as EDC. More often than not, NHS is added to form semi-stable reactive intermediate.
A typic recipe is as follows:
PBS buffer solution, containing 0.2 M EDC and 0.05 M NHS.
References:
1. http://en.wikipedia.org/wiki/Carbodiimide
2. http://www.piercenet.com/Objects/View.cfm?type=ProductFamily&ID=02030312
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